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An investigation into the catalytic deuteration and tritiation of dehydroproline9-buserelin

✍ Scribed by J. Oehlke; T. Brankoff; M. Schmidt; M. Brudel; U. Kertscher; H. Berger

Publisher
John Wiley and Sons
Year
1993
Tongue
French
Weight
439 KB
Volume
33
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

For optimizing the catalytic tritiation of peptides, the catalytic deuteration of buserelin and DhP^9^‐buserelin^1^ in DMA after reaction of DhP^9^‐buserelin were comparable in both solvents. The nonspecific incorporation of deuterium found after deuteration of buserelin was clearly lower after reaction in DMA. Tritiation of DhP^9^‐buserelin in DMA using 85% tritium gas, with conditions optimized by the deuteration experiments, resulted in a specific radioactivity of 1.3 TBq/mmol. 72 and 13 percent of the incorporated radioactivity were found to be associated with proline and histidine, respectively, after acidic hydrolysis.

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