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An introduction to quadrupole–time-of-flight mass spectrometry

✍ Scribed by Igor V. Chernushevich; Alexander V. Loboda; Bruce A. Thomson

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
492 KB
Volume
36
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

A brief introduction is presented to the basic principles and application of a quadrupole–time‐of‐flight (TOF) tandem mass spectrometer. The main features of reflecting TOF instruments with orthogonal injection of ions are discussed. Their operation and performance are compared with those of triple quadrupoles with electrospray ionization and matrix‐assisted laser desorption/ionization (MALDI) TOF mass spectrometers. Examples and recommendations are provided for all major operational modes: mass spectrometry (MS) and tandem MS (MS/MS), precursor ion scans and studies of non‐covalent complexes. Basic algorithms for liquid chromatography/MS/MS automation are discussed and illustrated by two applications. Copyright © 2001 John Wiley & Sons, Ltd.

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