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An incubation system for the NMR study of kidney tubules

✍ Scribed by H. Ammann; Y. Boulanger; P. Legault; P. Vinay

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
584 KB
Volume
12
Category
Article
ISSN
0740-3194

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✦ Synopsis

Isolated kidney cortical tubules require a very rapid oxygen supply and mechanical agitation to be optimally functional. A sample chamber in which a tubule suspension is oxygenated by recirculating oxygen gas inside a coil of dialysis fibers to avoid cell loss through bubbling and in which the tubules are agitated by a gas-driven turbine has been designed. In such a system, dog cortical tubules (35-45 mg/ml) were found to be metabolically stable for more than 3 h as indicated by linear lactate consumption and glucose production. Small pH variations resulting from carbon dioxide and bicarbonate productions were measured. Good-quality 23Na NMR spectra of dog kidney cortical tubules were recorded with such a system, allowing a I-min time resolution.

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