An in vitro clonal assay of adherent stem cells (ASC) in mouse marrow

✍ Scribed by Ursula Reincke; Margery Rosenblatt; Samuel Hellman

• Book ID: 102885889
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 982 KB
• Volume: 121
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041210203

✦ Synopsis

Hematopoietic stem cells with high proliferative capacity can be assayed when stromal bone marrow cultures are overlaid with limiting dilutions of marrow samples. This leads to hematopoietic growth after 4 weeks in a fraction of cultures, consistent with expectations based on Poisson statistics. It will be shown that monoclonal cultures are obtained that last from 2 to 15 weeks and that can generate u p to several million mature granulocytes. The originating clone-forming cell is named adherent stem cell (ASC) because of its adherence to plastic or stromal surfaces. The ASC is comparable to the CFU-S in frequency, proliferative capacity and in its ability to give rise to CFU-S. As an unexpected additional finding we report that a mode of "clonal succession" was apparent in cultures which expressed more than one clone.