## Abstract Three assays for bone marrow progenitor cells have been used to determine the effect of single doses of two cytotoxic agents, cyclophosphamide and vinblastine. The assays employed were the agar colony forming and spleen colony forming assays and the crythroid repopulating ability. In n
In vitro frequency analysis of spleen colony-forming and marrow-repopulating hemopoietic stem cells in the mouse
β Scribed by Rob E. Ploemacher; Johannes P. van der Sluijs
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 487 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1573-0603
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract In vitro macrophage colonyβforming cells (MβCFC) have been detected in bone marrow (BM) (317/10^5^ cells), spleen (SPL) (81/10^5^), and peripheral blood leukocytes (PBL) (242/10^5^) of the mouse. These MβCFCs were similar to those previously detected in thymus (T) (30/10^6^) and lymph n
An adaptation of a previously reported flow cytometric technique is described. This technique was applied to study the DNA distribution of mouse granulocyte-macrophage colony forming cells (GM-CFc) grown in a methyl cellulose culture system. This method involved the collection of cell clusters and c
## Abstract Erythropoietin (epo) added to liquid cultures of mouse bone marrow cells affected both the numbers of spleen colonyβforming cells (CFU) in the cultures and the types of spleen colonies formed from these cells in irradiated hosts. Epo caused an increase in the numbers of CFU detected in
## Abstract C~57~BL bone marrow cells were separated on the basis of their sedimentation velocity at unit gravity and cell fractions cultured in agar using three types of colony stimulating factor (CSF). Colonyβforming cells separated as a single peak (s = 4.4 mm/hr) in cultures stimulated by mouse