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An improved syringe technique for the preparation of oligonucleotides of defined sequence

✍ Scribed by H. Seliger; C. Scalfi; F. Eisenbeiss

Publisher: Elsevier Science
Year: 1983
Tongue: French
Weight: 327 KB
Volume: 24
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(01)99822-3

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✦ Synopsis

Resulting from furtherdevelopment of the technique described by Tanaka and Letsinger a simple syringe-based reaction system was assembled for efficient routine synthesis of oligonucleotides, and its applicability exemplified.

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A computer program has been developed to aid in the evaluation of strategies for the synthesis of oligodeoxyribonucleotides of defined sequence. The program reduces the time required for the development of the most cost-effective strategy from several hours to a few minutes. The chemist supplies the