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An Improved RNA Isolation Method for Filamentous FungusBlakeslea trisporaRich in Polysaccharides

✍ Scribed by Ye Li; Wenya Wang; Xueling Du; Qipeng Yuan

Book ID
107564980
Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
118 KB
Volume
160
Category
Article
ISSN
0273-2289

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In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 M guanidium thiocyanate, 25 mM sodium citrate, 0.5% Sarcosyl, 0.1 M beta-mercaptoethanol, pH 7.0, and then performing t