An improved micro enzyme sensor for bioprocess monitoring by flow injection analysis
β Scribed by S. Haemmerli; A. Schaeffler; A. Manz; H.M. Widmer
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 385 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0925-4005
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π SIMILAR VOLUMES
A flow injection analysis (FIA) enzyme sensor for sucrose is described. Two approaches were employed for the construction of the biosensor. In the first approach, the enzymes invertase and mutarotase were covalently coupled to controlled pore glass (CPG) particles and placed in a packed bed reactor.
Glutamate and glutamine were determined by lummol chemilummescence with flow-injection analysis (FIA) based on immobilized L-glutamate oxidase and glutaminase coupled with peroxidase The laboratory-made flowthrough cell of the detector has a measured volume of only 15 jA The hydrogen peroxide produc