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An Improved Method to Obtain Highly Differentiated Monolayers of Human Bronchial Epithelial Cells

โœ Scribed by Luis J. V. Galietta, Sabina Lantero, Andrea Gazzolo, Oliviero Sacco, Luca Romano, Giovanni A. Rossi and Olga Zegarra-Moran

Book ID
125624649
Publisher
Society for In Vitro Biology
Year
1998
Tongue
English
Weight
256 KB
Volume
34
Category
Article
ISSN
1475-2689

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Serially passaged normal human bronchial epithelial (NHBE) cell monolayers were established on Transwell inserts via an air-liquid interface (ALI) culture method. NHBE cells were seeded on polyester Transwell inserts, followed by an ALI culture from day 3, which resulted in peak TEER value of 766+/-