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An improved method to obtain highly differentiated monolayers of human bronchial epithelial cells

โœ Scribed by Luis J. V. Galietta; Sabina Lantero; Andrea Gazzolo; Oliviero Sacco; Luca Romano; Giovanni A. Rossi; Olga Zegarra-Moran

Book ID
107427588
Publisher
Society for In Vitro Biology
Year
1998
Tongue
English
Weight
418 KB
Volume
34
Category
Article
ISSN
1475-2689

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Air-liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies
โœ Hongxia Lin; Hong Li; Hyun-Jong Cho; Shengjie Bian; Hwan-Jung Roh; Min-Ki Lee; J ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 318 KB ๐Ÿ‘ 1 views

Serially passaged normal human bronchial epithelial (NHBE) cell monolayers were established on Transwell inserts via an air-liquid interface (ALI) culture method. NHBE cells were seeded on polyester Transwell inserts, followed by an ALI culture from day 3, which resulted in peak TEER value of 766+/-