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An improved method of DNA isolation suitable for PCR-based detection of begomoviruses from jute and other mucilaginous plants

โœ Scribed by Raju Ghosh; Sujay Paul; Subrata Kumar Ghosh; Anirban Roy


Book ID
113768044
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
260 KB
Volume
159
Category
Article
ISSN
0166-0934

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An improved method of plant megabase DNA
โœ Rod A. Wing; Vipin K. Rastogi; Hong-Bin Zhang; Andrew H. Paterson; Steven D. Tan ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 835 KB

The isolation of high quality megabase DNA from plant cells that is susceptible to a variety of molecular reagents is a critical first step in the physical analysis of complex genomes. A method for the isolation of such DNA by encapsulating plant protoplasts in agarose microbeads is presented. In co