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An improved DNA isolation method and PCR protocol for efficient detection of multicomponents of begomovirus in legumes

✍ Scribed by A. Rouhibakhsh; J. Priya; M. Periasamy; Q.M.I. Haq; V.G. Malathi


Book ID
113767647
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
158 KB
Volume
147
Category
Article
ISSN
0166-0934

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The isolation of high quality megabase DNA from plant cells that is susceptible to a variety of molecular reagents is a critical first step in the physical analysis of complex genomes. A method for the isolation of such DNA by encapsulating plant protoplasts in agarose microbeads is presented. In co