Determination of 25-Hydroxycholecalcifer
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Pirjo Mattila; Vieno Piironen; Esko Uusi-Rauva; Pekka Koivistoinen
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Article
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1993
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Elsevier Science
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English
โ 295 KB
A high-performance liquid chromatographic (HPLC) procedure for determining 25 -hydroxycholecalciferol contents in chicken egg yolk has been developed. The procedure consists of the following steps: saponification. extraction of the unsaponifiable fraction, purification using solidphase extraction, c