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An improved method for the preparation of cyclooctaamylose, using starches and the cyclodextrin glycosyltransferase of Klebsiella pneumoniae M 5 al

✍ Scribed by Hans Bender

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 548 KB
Volume: 124
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(83)88458-4

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✦ Synopsis

Larger amounts (18.7% of total carbohydrate) of cyclooctaamylose (cyclomaltooctaose) were produced from starches by the cyclodextrin glycosyltransferase {(l~4)-a-D-glUCan:[(1~4)-cY-D-glUCOpyranOSyl]tranSferaSC (cyclising), EC 2.4.1.19) of Klebsiellu pneumoniue M 5 al by digestion in 2mmM sodium acetate (pH 6.9) and the addition of bromobenzene after pre-incubation for 7 h. The dependence of the formation of cyclooctaamylose on the concentration of sodium acetate, initial concentration of substrate, and enzyme-substrate ratio has been studied.

A simple method for the preparation of pure cyclooctaamylose has been developed.

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