The actions of the wildtype and a truncated alpha-cyclodextrin glycosyltransferase from Klebsiella pneumoniae strain M 5 al on malto-oligosaccharides showed no significant differences, and there was marked dependence of the kinetic parameters on the chain lengths of the substrate. The action of the
Branched saccharides formed by the action of his-modified cyclodextrin glycosyltransferase from Klebsiella pneumoniae M 5 al on starch
โ Scribed by Hans Bender
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 353 KB
- Volume
- 222
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
Digestion of potato starch with His-modified alpha-cyclodextrin glycosyltransferase from Klebsiella pneumoniae M 5 al yielded branched tetra- to nona-saccharides, as revealed by debranching with pullulanase. Maltose and maltotriose stubs preponderated together with small proportions of D-glucose stubs. The branched saccharides accounted for approximately 1.2% of the starch.
๐ SIMILAR VOLUMES
The analysis of the (1 leads to 4)-alpha-D-glucopyranosyl transfer-products from some linear and cyclic substrates by quantitative h.p.l.c. illuminated the mode of action of the cyclodextrin glycosyltransferases [1 leads to 4)-alpha-D-glucan:[(1 leads to 4)-alpha-D-glucopyranosyl]transferase (cyclis