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An improved method for the preparation of 5′-monophosphoderivatives of the common ribonucleosides

✍ Scribed by J.L. Darlix; H.P.M. Fromageot; P. Fromageot

Book ID
115742547
Publisher
Elsevier Science
Year
1967
Weight
171 KB
Volume
145
Category
Article
ISSN
0005-2787

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Pyridoxal Y-phosphate was synthesized by first oxidizing [Wlpyridoxine to [Wlpyridoxal with MnOp ["C]Pyridoxal, isolated by cation-exchange chromatography, was then reacted with ATP in the presence of rat liver pyridoxal kinase to form ["C]pyridoxal Y-phosphate. The rat liver enzyme was used instead