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An improved method for detection and quantification of chitinase activities

✍ Scribed by Guthrie, Jennifer L; Khalif, Sagal; Castle, Alan J

Book ID
120618419
Publisher
NRC Research Press
Year
2005
Tongue
French
Weight
264 KB
Volume
51
Category
Article
ISSN
0008-4166

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We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld