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A gel diffusion assay for visualization and quantification of chitinase activity

โœ Scribed by Xiaohong Zou; Hiroyuki Nonogaki; Gregory E. Welbaum

Book ID
111640283
Publisher
Humana Press Inc
Year
2002
Tongue
English
Weight
126 KB
Volume
22
Category
Article
ISSN
1073-6085

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โœ Bruce Downie; Lynnette M.A. Dirk; Kristen A. Hadfield; Thea A. Wilkins; Alan B. ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 602 KB

Increased binding of ruthenium red to pectin as the number of methyl esters attached to the pectin decreases was used as the basis for a gel diffusion assay for pectin methylesterase (PME, EC 3.1.1.11) activity. The stained zone diameters resulting from the hydrolysis of 0.1% (w/v) 90% esterified pe