An improved method for counting tritium and carbon-14 in acrylamide gels
โ Scribed by Ross S. Basch
- Book ID
- 107710857
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 312 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %
Electrophoresis in polyacrylamide gels is widely used for analysis and characterization of proteins and mixtures of proteins. It is frequently necessary to obtain estimates of the radioactivity of protein bands in the gels, and of the relative amounts of two radionuclides, generally 3H ,and l\*C. Ca