An improvement in a procedure for counting tritium and carbon-14 in polyacrylamide gels
β Scribed by Milton Zaitlin; V. Hariharasubramanian
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 122 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
Electrophoresis in polyacrylamide gels is widely used for analysis and characterization of proteins and mixtures of proteins. It is frequently necessary to obtain estimates of the radioactivity of protein bands in the gels, and of the relative amounts of two radionuclides, generally 3H ,and l\*C. Ca
Large (200 mm3) slices of polyacrylamide gels crosslinked with N,N'-diallyltartardiamide which contain tritium-labeled protein are readily solubilized in periodic acid for liquid scintillation counting of radioactivity, but the apparent recovery of label never exceeds 82%. Extraction of the slices w