## Abstract Analysis of trimethylsilylethers of methyl esters of bile acids exhibits high separation efficiency with a 20 m Γ 0.3 mm glass capillary column covered with a crystal layer of barium carbonate and coated with polyethyleneglycol 20000 as liquid phase [3]. The sensitivity and reproducibil
An improved column for gas-liquid chromatography of substituted cholanic acids
β Scribed by Tatsuo Okishio; Padmanabhan P. Nair
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 225 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
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## Abstract The proposed combination of a rather simple procedure for sample preparation with capillary gasβliquid chromatography using a barium carbonate/polyethyleneglycol 20,000 column and a Grobβtype onβcolumn injector permits measurement of bile acids in serum with high separation efficiency,
The extract was filtered and the filtrate diluted with an equal volume of water. 50 ml strong solution of lead subacetate was added and the mixture centrifuged after 5 minutes. Excess lead was precipitated with 10% disodium hydrogen phosphate and the mixture again centrifuged. The supernatant was su
## Abstract A carefully Standardized technique is described for the preparation of glass capillary columns which can be used successfully for routine quantitative amino acid analysis. Comparison is made between two different modes of sample injection. Preliminary quantitative results from βsplitβ i