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An improved cathepsin-D substrate and assay procedure

✍ Scribed by Nirankar Agarwal; Daniel H. Rich

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
544 KB
Volume
130
Category
Article
ISSN
0003-2697

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✦ Synopsis

Ten analogs of the peptide A-Phe(NO+Phe-Val-Leu-B were synthesized and tested as sub strates for cathepsin D and pepsin. The best substrate found for cathepsin D, Phe-Ala-Ala-Phe(NO,)-Phe-Val-Leu-OM4P

(& = 2.9 s-l; K,,, = 7.1 PM), has the largest k&K,,, value (408 mM-' s-r) reported to date for this enzyme. The effect of peptide structure on solubility and kinetic parameters is discussed. The peptide provides a useful new substrate for continuous assay of cathepsin D.

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