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An improved HPLC assay of the substrate and products of the isopenicillin N synthase

✍ Scribed by Boudewijn D. Meesschaert; Jesús Cortés; Emilio Alvarez; Paloma Liras; Juan F. Martín

Publisher: Springer-Verlag
Year: 1987
Tongue: English
Weight: 251 KB
Volume: 1
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00159337

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✦ Synopsis

An improved HPLC method is described to separate isopenicillin N from its penicilloic acid and from the reduced and oxidized forms of d -(L-oc.-aminoadipyl)-L-cysteinyl-D-valine and of DTT. When applied to assay the activity of isopenicillin N synthase only isopenicillin N and its penicilloic acid could be detected.

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