We have developed an improved assay method to measure sphingomyelinase activity and to detect this enzyme separated on polyacrylamide gels. The assay of sphingomyelinase activity involved immobilizing \(\left[\mathbf{N}\right.\)-methyl \(\left.{ }^{14} \mathrm{C}\right]\) sphingomyelin on polyvinyld
An improved assay for measuring the antimotility activity of chemical agents towards spermatozoa
β Scribed by R.G. Laughlin; J.R. Innis; G.E. Ficken
- Book ID
- 115843435
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 469 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0010-7824
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
While the L-arginine conversion assay has been utilized to measure nitric oxide synthase (NOS) activity in isolated enzyme and pure cell preparations, this method often fails to provide accurate measurements in whole tissues. Biological tissues contain variable amounts of unlabeled substrate and enz
The internalization of fluorescent 7-nitro-2,1,3-benzoxadiazol-4-yl (NBD)-phospholipids from the plasma membrane can be assessed by the irreversible quenching of analogues in the outer leaflet by dithionite. Here we have utilized this assay to follow the redistribution of short-chain C6-NBD-sphingom