𝔖 Bobbio Scriptorium
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An immunofluorescent method using crithidia luciliae to detect antibodies to double-stranded DNA

✍ Scribed by William Crowe; Irving Kushner

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
748 KB
Volume
20
Category
Article
ISSN
0004-3591

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✦ Synopsis

Abstract

The immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double‐stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. This method was less sensitive than a Millipore filter method for detecting antibodies to DNA. It was positive only in subjects with systemic lupus erythematosus or drug‐induced antinuclear factors. This technique appears suitable for study of the immunochemical characteristics of antibodies to dsDNA.

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A simple and sensitive enzyme-linked immunosorbent assay (ELISA) is described for the measurement of antibodies to native DNA (double-stranded) and to heat-denatured DNA (single-stranded) in sera of patients with connective tissue disorders. DNA bound to polyvinylchloride plates is incubated with se