Structural requirements of DNA used in the Farr assay to detect antibodies directed against double-stranded DNA

Specificity in systemic lupus erythemato

Specificity in systemic lupus erythematosus of antibodies to double-stranded dna measured with the polyethylene glycol precipitation assay

✍ Ruud Smeenk; Gonnie Van Der Lelij; Tom Swaak; Janny Groenwold; Lucien Aarden 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 740 KB

Recently, a new radioimmunoassay-the polyethylene glycol (PEG) assay-was introduced to measure antibodies to double-stranded (ds) DNA. In this method, polyethylene glycol precipitation of formed 3H-DNMantiDNA complexes is used instead of the ammonium sulfate precipitation used in the Farr assay. In