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An HPLC assay for rat liver ferrochelatase activity

โœ Scribed by Famei Li; Chang Kee Lim; Timothy J. Peters


Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
452 KB
Volume
2
Category
Article
ISSN
0269-3879

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A simple and economical method of assaying rat liver microsomal estrone and testosterone glucuronyltransferase activity has been developed. Liver microsomes were activated by pretreatment with Lubrol WX. The incubation was carried out at 37ยฐC for 30 min and contained 30-600 PM steroid, l-2 mM UDP-gl