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An evaluation of the agargel electrophoretic method for the analysis of specific steroid binding proteins

✍ Scribed by Alun Hughes; H.I. Jacobson; R.K. Wagner

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
428 KB
Volume
78
Category
Article
ISSN
0003-2697

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✦ Synopsis

This paper presents data on the reproducibility of the agargel electrophoretic method for the analysis of specific steroid binding proteins, together with an evaluation of the auxiliary techniques required to produce a suitable assay sample. We find for intra-assay variability a coefficient of variation of approximately 3%, while for the interassay variability the value is approximately 7%. The method is also shown capable of assessing and correcting for the dissociation of the steroid-protein complexes during analysis. In view of the current preoccupation with the clinical utility of receptor assays, we feel that similar data should be made available for the other commonly used receptor assays.

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