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An ESR and HPLC-EC Assay for the Detection of Alkyl Radicals

✍ Scribed by Novakov, Christo P.; Feierman, Dennis; Cederbaum, Arthur I.; Stoyanovsky, Detcho A.

Book ID
120356785
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
113 KB
Volume
14
Category
Article
ISSN
0893-228X

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An assay method is described for measuring the catalytic activity of glutamine:5-phosphoribosyl-1-pyrophosphate amidotransferase (EC 2.4.2.14), the first enzyme in the de novo purine biosynthetic pathway. Phosphoribosylamine, the unstable product of the enzymatic reaction, is trapped by glycinamide