A new type of enzymatic sensors for the determination of urea based on an ionophore-free pH membrane electrode has been constructed. The membrane material was polyvinyl chloride modified with 1,4-diaminobutane. Chemical urease immobilization enabled the formation of a mono-and bimolecular enzyme la
An Enzyme microsensor for urea based on an ammonia gas electrode
β Scribed by Jose P. Joseph
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 513 KB
- Volume
- 169
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
A urea enzyme microsensor was fabricated by immobilizing urease at the tip (lo-rm diameter) of a rapidly responding ammonia gas microelectrode based on antimony. The construction and evaluation of both the urea sensor and the ammonia electrode are described in detail. The urea sensor responds to lo-'-lo+ M urea in 30-45 S.
π SIMILAR VOLUMES
The pH response of a pH-sensitive fieldeffect transistor (FET) is not affected by a ca. l-pm thick membrane formed from r-aminopropyltriethoxysilane and glutaraldehyde over the gate insulator by a vapor deposition method. The response between pH 5.5 and 8.5 is ca. 61 mV pH-' at 37Β°C in 5 mM Tris-HCl
## Abstract The preparation of gas diffusion electrodes and their use in an amperometric enzyme biosensor for the direct detection of a gaseous analyte is described. The gas diffusion electrodes are prepared by covering a PTFE membrane (thickness 250β ΞΌm, pore size 2β ΞΌm, porosity 35%) with gold, pla