𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An enzyme-linked immunosorbent assay to quantitate the elastin crosslink desmosine in tissue and urine samples

✍ Scribed by Zeenat Gunja-Smith

Book ID
102987208
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
574 KB
Volume
147
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

An enzyme-linked immunosorbent assay method has been developed for the determination of desmosine. The method is based on an inhibition immunoassay (under nonequilibrium conditions) and uses rabbit antisera directed against a desmosine-bovine serum albumin conjugate and microtiter plates coated with desmosine-gelatin conjugate. The assay quantitates desmosine in the range 2.5-50 pmol in tissue and urine samples. Important applications of this rapid and sensitive assay are in studying elastin metabolism and in screening for monoclonal antibodies against desmosine. Methods are described for obtaining a constant level ofsubstitution of desmosine per molecule of bovine serum albumin and for preparing a desmosine-gelatin coating antigen. Five different antibody preparations directed against desmosine exhibit 15-20s cross-reactivity toward pyridinohne (3-hydroxypyridinium), a nonreducible collagen crosslinking compound also present in urine and many tissue samples. 0 1985 Academic PW, IW.

📜 SIMILAR VOLUMES

Development and validation of an enzyme-
Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio)
✍ François Brion; Bente M. Nilsen; Janne K. Eidem; Anders Goksøyr; Jean Marc Porch 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 910 KB

## Abstract In this study, an enzyme‐linked immunosorbent assay (ELISA) was developed to quantify vitellogenin (Vtg) in zebrafish (__Danio rerio__). Zebrafish Vtg (zf‐Vtg) was purified from whole‐body homogenates of estradiol‐exposed zebrafish, and polyclonal antibodies against zf‐Vtg were raised.

Development of an enzyme-linked immunoso
Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y
✍ Cara Empey Campora; Yoshitsugi Hokama; Kenichi Yabusaki; Minoru Isobe 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 168 KB

## Abstract A sandwich enzyme‐linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G–hor‐seradish peroxidase conjugate specific to the

Comparative quantitation of abscisic aci
Comparative quantitation of abscisic acid in plant extracts by gas-liquid chromatography and an enzyme-linked immunosorbent assay using the avidin-biotin system
✍ B. Leroux; R. Maldiney; E. Miginiac; L. Sossountzov; B. Sotta 📂 Article 📅 1985 🏛 Springer-Verlag 🌐 English ⚖ 560 KB

In this report we describe an enzymelinked immunosorbent assay (ELISA) for the quantitation of abscisic acid (ABA) in plant extracts. A microtitration plate is coated with an ABA-protein complex. The ABA, standard or sample, is then added to each well with a limiting quantity of rabbit anti-ABA anti