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An enzymatic method for measurement of ascorbate-2-phosphate

✍ Scribed by T.A. Bensinger; T.F. Zuck; B. Tolbert; S. McLaughlin; K. Aguirre; C.C. Peck; M. Knight


Book ID
115766677
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
820 KB
Volume
19
Category
Article
ISSN
0006-2944

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An enzymatic method for the measurement
✍ F.A.M. de Azeredo; G.K. Feussner; W.D. Lust; J.V. Passonneau πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 598 KB

A method is described for the analysis of GTP/GDP, or GDP alone, in a coupled enzymatic assay. Samples are pretreated with creatine kinase in the presence of phosphocreatine to remove the ADP present. Succinyl CoA synthetase is used to convert GTP to GDP in the presence of succinate and coenzyme A.