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An enzymatic cycling method for the measurement of myo-inositol in biological samples

โœ Scribed by Takuji Kouzuma; Mamoru Takahashi; Teruo Endoh; Reiko Kaneko; Nobuyuki Ura; Kazuaki Shimamoto; Naoki Watanabe

Book ID
117074385
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
135 KB
Volume
312
Category
Article
ISSN
0009-8981

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๐Ÿ“œ SIMILAR VOLUMES

An enzymatic method for the measurement
An enzymatic method for the measurement of GTP and GDP in biological samples
โœ F.A.M. de Azeredo; G.K. Feussner; W.D. Lust; J.V. Passonneau ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 598 KB

A method is described for the analysis of GTP/GDP, or GDP alone, in a coupled enzymatic assay. Samples are pretreated with creatine kinase in the presence of phosphocreatine to remove the ADP present. Succinyl CoA synthetase is used to convert GTP to GDP in the presence of succinate and coenzyme A.