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An electrophoretic method for the determination of the proportion of γ-aminobutyric acid in a mixture of labeled neurotransmitter and its catabolites

✍ Scribed by A. Cupello; M.V. Rapallino; G. Besio; P. Mainardi


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
251 KB
Volume
160
Category
Article
ISSN
0003-2697

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✦ Synopsis


An electrophoretic method for the separation of y-aminobutyric acid (GABA) from its rnetabelites after GABA-transaminase attack is presented. The method is based on the fact that at neutral pH GABA has no net electrical charge, whereas its major metabolites, succinic acid and Krebs cycle intermediates, are negatively charged. The method appears to be especially suitable for evaluation of true-labeled neurotransmitter within the radioactivity which is found in synaptosomes after labeled GABA-uptake studies.


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