An electrophoretic method for the determination of the proportion of γ-aminobutyric acid in a mixture of labeled neurotransmitter and its catabolites
✍ Scribed by A. Cupello; M.V. Rapallino; G. Besio; P. Mainardi
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 251 KB
- Volume
- 160
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
An electrophoretic method for the separation of y-aminobutyric acid (GABA) from its rnetabelites after GABA-transaminase attack is presented. The method is based on the fact that at neutral pH GABA has no net electrical charge, whereas its major metabolites, succinic acid and Krebs cycle intermediates, are negatively charged. The method appears to be especially suitable for evaluation of true-labeled neurotransmitter within the radioactivity which is found in synaptosomes after labeled GABA-uptake studies.
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