An electrometric method for the estimation of sucrose and reducing sugars

✍ Scribed by Coalstad, S. E.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1946
• Weight: 366 KB
• Volume: 65
• Category: Article
• ISSN: 0368-4075
• DOI: 10.1002/jctb.5000650804

✦ Synopsis

Abstract

The writer has adapted the Dead‐Stop End Point method of titration evolved by C. W. Foulk and A. T. Bawden in 1926 to the volumetric estimation of reducing sugars with Fehling's Solution without previous clarification of the sugar solutions. The end point is detected by the polarization effect at two plain copper electrodes dipping into the solution being titrated, to which an E.M.F. of 10 millivolts is applied. With impure sugar solutions, the impurities act as depolarizing agents, but the writer has overcome this effect by adding 0.3 gram of purified animal charcoal to the solution at the start of the titration. A number of examples is given.