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An electrochemical assay for tissue plasminogen activator (t-PA)

✍ Scribed by Marco Cardosi; Wah On Ho; Dale Athey; Calum McNeil

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 604 KB
Volume: 4
Category: Article
ISSN: 1040-0397
DOI: 10.1002/elan.1140040305

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✦ Synopsis

Abstract

An electrochemical assay for tissue plasminogen activator (t‐PA) is described. The activity of t‐PA is measured in a coupled assay following the amidolytic cleavage of a synthetic tripeptide by the enzyme plasmin. The released electroactive label, p‐aminophenol, is detected anodically either at a glassy carbon indicator electrode or at a platinized activated carbon electrode in a specially constructed perspex cell. Calibration plot data for t‐PA are presented for buffered samples using the perspex block‐type cell. The limit of detection for t‐PA in this assay is 0.25 IU/ml. In addition, the assay format is adapted to yield kinetic information using repetitive square wave voltammetry as the mode of detection.

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