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An efficient synthesis of polyguanylic acid by a thermophilic polynucleotide phosphorylase

✍ Scribed by Yo Kikuchi; Keiko Hirai; Fumio Hishinuma; Kenji Sakaguchi

Book ID
115746778
Publisher
Elsevier Science
Year
1977
Weight
450 KB
Volume
476
Category
Article
ISSN
0005-2787

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Synthesis of a novel fluorescent polyrib
Synthesis of a novel fluorescent polyriboadenylic acid analog by polynucleotide phosphorylase
✍ K. C. Tsou; K. F. Yip πŸ“‚ Article πŸ“… 1974 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 278 KB

## Abstract Poly(1,__N__^6^‐etheno‐2‐aza‐adenylic acid) [poly(2‐aza‐ΡA)] was synthesized from 1,__N__^6^‐etheno‐2‐aza‐adenosine 5′‐diphosphate (2‐aza‐ΡADP) and __Escherichia coli__ polynucleotide phosphorylase. The values __K__~__m__~ = 1.02 m__M__, __V__ = 1.06 ΞΌmol hr^βˆ’1^ enzyme unit^βˆ’1^ were fou