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Synthesis of a novel fluorescent polyriboadenylic acid analog by polynucleotide phosphorylase

✍ Scribed by K. C. Tsou; K. F. Yip

Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
278 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Poly(1,N^6^‐etheno‐2‐aza‐adenylic acid) [poly(2‐aza‐εA)] was synthesized from 1,N^6^‐etheno‐2‐aza‐adenosine 5′‐diphosphate (2‐aza‐εADP) and Escherichia coli polynucleotide phosphorylase. The values K~m~ = 1.02 m__M__, V = 1.06 μmol hr^−1^ enzyme unit^−1^ were found for the polymerization reaction. In contrast to polyadenylic acid, this novel fluorescent polymer has a random structure in solution. The application of the 2‐aza‐εADP for localization of polynucleotide phosphorylase was also described.

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