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An efficient genetic transformation method for glycerol producer Candida glycerinogenes

โœ Scribed by Xianzhong Chen; Huiying Fang; Zhiming Rao; Wei Shen; Bin Zhuge; Zhengxiang Wang; Jian Zhuge


Book ID
113799826
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
325 KB
Volume
163
Category
Article
ISSN
0944-5013

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## Abstract In order to genetically modify microorganisms capable of producing polyhydroxyalkanoate (PHA) biopolymers, a simple and rapid method to prepare freshly plated __Pseudomonas__ cells for transformation via electroporation was developed. This method can be used to transfer both replicative