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An efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria

โœ Scribed by Fausta Serafini; Francesca Turroni; Simone Guglielmetti; Laura Gioiosa; Elena Foroni; Valentina Sanghez; Alessandro Bartolomucci; Mary O'Connell Motherway; Paola Palanza; Douwe van Sinderen; Marco Ventura

Book ID
114777931
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
206 KB
Volume
333
Category
Article
ISSN
0378-1097

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## Abstract In order to genetically modify microorganisms capable of producing polyhydroxyalkanoate (PHA) biopolymers, a simple and rapid method to prepare freshly plated __Pseudomonas__ cells for transformation via electroporation was developed. This method can be used to transfer both replicative