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An easy method to determine the kinetic parameters of bipasic reactions

✍ Scribed by AM Kayastha; AK Gupta


Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
93 KB
Volume
15
Category
Article
ISSN
0307-4412

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An Isothermal Titration Calorimetric Met
✍ Lifeng Cai; Aoneng Cao; Luhua Lai πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 English βš– 68 KB

An isothermal titration calorimetric (ITC) method was developed to measure the kinetic parameters of ribonuclease A catalytic hydrolysis of cytidine 2',3'-cyclic monophosphate. Employing the inhibition of product as a probe, the K(m), K(i), k(c), and DeltaH(m) can be determined by two simple calorim