A New Quantitative Method of Real Time R
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Weihong Liu; David A. Saint
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Article
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2002
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Elsevier Science
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English
β 108 KB
Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference