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An Easier Method for Locating Replication Origins Based on Real-Time Polymerase Chain Reaction

✍ Scribed by Y. Mei; D. Chen; D. Sun; Y. Yang; Y. Huang; P. Shen; X. Chen


Publisher
Springer
Year
2008
Tongue
English
Weight
215 KB
Volume
56
Category
Article
ISSN
0343-8651

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Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference