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An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’

✍ Scribed by Michel E. Goldberg; Gennady V. Semisotnov; Bertrand Friguet; Kunihiro Kuwajima; Oleg B. Ptitsyn; Shintaro Sugai


Book ID
115923516
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
835 KB
Volume
263
Category
Article
ISSN
0014-5793

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Kinetic characterization of early interm
✍ Sylvie Blond; Dr. Michel E. Goldberg 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 841 KB

This report describes the use of fluorescence energy transfer between an intrinsic energy donor (tryptophan 177) and two chemically added acceptors to study intermediates in the folding of the beta 2 subunit of E. coli tryptophan-synthase. Two early folding steps are thus identified and characterize