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Transient Non-Native Interactions in Early Folding Intermediates Do Not Influence the Folding Kinetics of Escherichia Coli Tryptophan Synthase β2 Subunits

✍ Scribed by Thierry Planchenault; Amiel Navon; Andreas J. Schulze; Michel E. Goldberg


Book ID
115132170
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
735 KB
Volume
240
Category
Article
ISSN
1432-1327

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✍ Sylvie Blond; Dr. Michel E. Goldberg 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 841 KB

This report describes the use of fluorescence energy transfer between an intrinsic energy donor (tryptophan 177) and two chemically added acceptors to study intermediates in the folding of the beta 2 subunit of E. coli tryptophan-synthase. Two early folding steps are thus identified and characterize