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An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays

โœ Scribed by Ralf G. Dietzgen; Roy E. Snoke; Bruce Babb

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
470 KB
Volume
164
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A calorimetric reagent, 4-(4'~nitro-2'-methylsulfonylphenylazo)phenyl phosphate (NMPP), has been shown to be an effective substrate of alkaline phosphatase. NMPP and p-nitrophenyl phosphate were applied in comparative studies using enzyme immunoassays for the detection of viral antigens and antiviral antibodies. The new substrate exhibited similar, or even higher, sensitivity than p-nitrophenyl phosphate depending on the substrate concentrations used. Positive and negative reactions were easier to define, even without cumbersome equipment. The enzyme reaction was terminated by the uncompetitive inhibitor, theophylline.

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