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An attempt at sperm-mediated gene transfer in mice and chickens

✍ Scribed by Gavora, J. S.; Benkel, B.; Sasada, H.; Cantwell, W. J.; Fiser, P.; Teather, R. M.; Nagai, J.; Sabour, M. P.

Book ID: 111672981
Publisher: Agricultural Institute of Canada
Year: 1991
Tongue: English
Weight: 334 KB
Volume: 71
Category: Article
ISSN: 0008-3984
DOI: 10.4141/cjas91-037

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We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin