An assay for thyroid hormone binding to chromatin receptors

✍ Scribed by Donald J. Gruol

• Book ID: 102983198
• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 522 KB
• Volume: 101
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(80)90204-3

✦ Synopsis

I have measured the interaction of T3 with highly soluble, expanded, rat liver chromatin using a new assay for the study of hormone binding to nucleoprotein. Bound hormone and free hormone were rapidly and quantitatively separated by the adsorption of the hormonenucleoprotein complex onto hydroxylapatite. This procedure satisfies several criteria for a successful binding assay: (1) The binding capacity is stable throughout the time required to reach equilibrium, (2) the ratio of specific to nonspecific binding (signal/noise) is at least 20: 1, (3) large numbers of samples can be handled easily, (4) the amount of bound hormone is directly proportional to the quantity of chromatin employed, (5) the hormone and its analogs display a range of affinities for the binding site, and (6) the binding occurs to a limited number of sites, over a free hormone concentration range which is similar to the hormone concentrations found in vivo.