✦ LIBER ✦

An assay procedure for solubilized thyroid hormone receptor: Use of lipidex

✍ Scribed by Qiulin Li; Naoki Yamamoto; Akira Inoue; Seiji Morisawa

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 424 KB
Volume: 192
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90198-3

No coin nor oath required. For personal study only.

✦ Synopsis

A method using Lipidex-1000 is reported for the assay of thyroid hormone receptor activity. The receptor was extracted from rat liver nuclei, incubated with [125I]T3, and applied to 1-ml disposable pipet tip columns containing a small volume of Lipidex. The resin absorbed the free hormone, and the receptor-bound hormone was recovered in the eluate. The method allowed accurate assay of the receptor activity, satisfying a linear relationship between the activity and the receptor protein concentration. The usefulness of this method was demonstrated by determining the pH optimum and the Kd and Bmax of T3 binding by the receptor. The Lipidex column can be used for the preparation of an unoccupied receptor after dissociation of the endogenously bound hormone.

📜 SIMILAR VOLUMES

Development of an immunoradiometric assa

Development of an immunoradiometric assay (IRMA) kit for determination of thyroid stimulating hormone in dry blood spot of newborns

✍ Virginia N. N. Borza; Elena V. Neacsu; Cristian I. Postolache; Lidia C. Matei 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 French ⚖ 62 KB

Stable Expression of a Synthetic Gene fo

Stable Expression of a Synthetic Gene for the Human Motilin Receptor: Use in an Aequorin-Based Receptor Activation Assay

✍ Christopher W. Carreras; Michael A. Siani; Daniel V. Santi; Susan B. Dillon 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 88 KB

A synthetic gene for the human motilin receptor containing 33 unique restriction sites was designed and stably coexpressed in HEK293 cells with the bioluminescent Ca 2؉ indicator protein aequorin. The dosedependent response of the receptor to motilin was demonstrated using transient transfections, a

Development of an assay for modulating a

Development of an assay for modulating anti-acetylcholine receptor autoantibodies using human rhabdomyosarcoma cell line

✍ Bradley W. Lyons; Lily L. Wu; Mark E. Astill; James T. Wu 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 70 KB 👁 1 views

Three types of autoantibodies against the acetylcholine receptors (AChR) of skeletal muscle are detectable in patients with myasthenia gravis including binding, blocking, and modulating anti-AChR antibodies. Modulating autoantibodies correlate best with the severity of the disease, but are also tech