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An assay for glucose 6-phosphatase based on the formation of glucose

✍ Scribed by Clark Bublitz

Book ID
104675363
Publisher
Springer
Year
1991
Tongue
English
Weight
252 KB
Volume
108
Category
Article
ISSN
0300-8177

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✦ Synopsis

A rapid, convenient method for the assay of glucose 6-phosphatase dependent on the removal of radioactive substrate from radioactive product by Dowex 2 fluoride is described. The enzymatic reaction is stopped by the addition of an ethanolic slurry of the resin. After the tubes are shaken, the radioactivity of glucose in the clarified supernatant layer is measured. The release of glucose is directly proportional to time and enzyme concentration. Detergents do not interfere with the method.

πŸ“œ SIMILAR VOLUMES

A continuous spectrophotometric assay fo
A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase
✍ Maria Stio; Paolo Vanni; Giancarlo Pinzauti πŸ“‚ Article πŸ“… 1988 πŸ› Elsevier Science 🌐 English βš– 484 KB

A continuous spectrophotometric assay for glucose 6-phosphatase is described. The method uses glucose dehydrogenase and mutarotase as ancillary enzymes. Glucose 6-phosphatase activity is measured by following NADH formation at 340 nm. The method is linear, at least up to 38 mU in the test which corr