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An arabinogalactan from the culture medium of Rubus fruticosus cells in suspension

✍ Scribed by Nicole Cartier; Gérard Chambat; Jean-Paul Joseleau

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
529 KB
Volume
168
Category
Article
ISSN
0008-6215

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✦ Synopsis

A water-soluble arabinogalactan, isolated from the extracellular medium of suspension-cultured cells of Rubus fruticosus, contained arabinose, rhamnose, galactose, and also protein (6.5%) and uranic acid (2.5%). Methylation analysis of the arabinogalactan and the arabinose-free product obtained by mild acid hydrolysis showed that the polysaccharide was a typical arabino3,6-galactan in which rhamnose and glucuronic acid occupied non-reducing terminal positions. Successive Smith-degradations combined with methylation analysis and i3C-n.m.r. spectroscopy revealed that the arabinogalactan contained a main chain cf (1+3)linked &D-galactopyranosyl residues with a high degree of branching at positions 6 by (l-6)-linked D-galactopyranosyl side-chains of various lengths, in which several contiguous residues were substituted at positions 3. The polymer is thus an arabinogalactan-protein belonging to the galactans of Type !I.

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